A CRITICAL EVALUATION OF ELISA TESTS USED TO DETECT ANTIBODIES AGAINST CAMPYLOBACTER IN HUMANS
Abstract:
Campylobacter infection is one of the most common causes of foodborne illness in the European Union. It is frequently followed by different sequelae, such as neuropathies and reactive arthritis. Diagnosis is made mainly by stool culture which means a lot of work, time and money. ELISAs, as serological tests are well suited for diagnosis’s sequelae or for identifying infection from stool sample with non-viable bacteria. At present, international consensus regarding ELISAs for campylobacter in humans exists. It was identified 17 studies validating such assays in a literature review. The best assay that was validated is the one developed “in-house” and which was used especially for research purposes, then for routine diagnosis. Taken into account the burden of the disease and the possible severity of campylobacter infection, it would be useful, for surveillance and diagnosis, to develop a standardized and commercially available ELISA assay.
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