IMPLANTATION OF VALVULAR COLLAGEN BIOMATERIALS SEEDEDWITH AUTOLOGOUS STEM CELLS – INTRAOPERATIVE HEMODYNAMIC MEASUREMENTS IN AN ANIMAL MODEL
Abstract:
Objective: achieving a pulmonary valve xenograft with no hemodynamic impact. Methods: In the Tissue Engineering and Regenerative Medicine Laboratory at the University of Medicine and Pharmacy Tîrgu-Mureş, acellular scaffolds were created from decellularized porcine pulmonary valves using mechanical, chemical and enzymatic methods. Those were included in a decellularized bovine pericardium conduct and placed in extra-anatomic position between the right ventricle outflow tract and the pulmonary trunk. 7 juvenile sheep about 4-5 months (22-25 kg) were selected as animal model. Right ventricle and pulmonary artery invasive pressures were measured intra-operatorily, before and after the ligature of the native pulmonary trunk. Systemic blood pressure, heart rate and oxygen saturation were also monitored. Results: The physiologic average of the right ventricle mean pressure was 20.14 mmHg (interval between 17 and 22) and in the pulmonary artery, it was 23.42 mmHg (interval between 21 and 26). Redirecting the blood flow through the valvular conduit, by ligating the pulmonary trunk origin produced similar values: 21.42 mmHg (19 to 23) in the right ventricle and 23.14 mmHg (21 to 26) in the pulmonary artery. The new means were not significantly different from the native values, as shown by the two-tailed p values of the paired t-test (p = 0.06, respectively 0.77, at a 95% confidence level). Arterial blood pressures were maintained between 110-130/70-80 mmHg, heart rate below 100/min and oxygen saturation above 94%. Conclusions: Our pulmonary valve xenograft implantation technique did not modify intraventricular and pulmonary pressures. Systemic blood pressure, heart rate and oxygen saturation were also stable. Long term follow-up is necessary to evaluate the xenograft performance.
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