FLOW CYTOMETRY’S CONTRIBUTION TO THE DIAGNOSIS OF THE MALIGNANT HAEMOPATHIES
Abstract:
Flow cytometry represents an indispensable examination for the malignant haemopathies diagnosis. The existence of an increasing number of fluorochromes in combination with the use of different lasers greatly enhances the versatility of flow cytometry. In myeloid acute leukaemias, CD33, MPO, and CD13 are the most frequently expressed antigens, followed by CD65s and CD15. The most frequently expressed antigens in B-lineage of acute lymphoblastic leukaemias are cyCD79a and HLA-DR, and in T-lineage of the same disease are cyCD3 and CD7. For the diagnosis of chronic lymphocytic leukaemia, the following antigens are useful: CD5, CD19, CD20, CD23, HLA-DR. Many markers are associated with an unfavourable prognosis in certain neoplasms, and some antibodies against lymphocytic and myelocytic antigens have been used effectively for the treatment of lymphoma and leukaemia.
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