NEWS IN THE DIAGNOSIS OF THE OCULAR TOXOPLASMOSIS
Abstract:
Biological, serologic, histological or molecular assays are used for the diagnosis of Toxoplasma gondii. There are a lot of serologic tests for the detection of Toxoplasma gondii, but they are still not enough. New research continues to be done. We consider the IgG avidity test as a new valuable method for the diagnosis of a recent infection. A rapid, sensitive, DNA quantitative reaction is the polymerase chain reaction (PCR) with fluids or tissues, including the amniotic liquid. Real-time PCR has recently been introduced for the diagnosis of toxoplasmosis. It combines the steps of amplification and PCR product detection in a single phase, thereby shortening the turnaround time from 24 to 48 h to less than 4 h. DNA extraction methods have also recently been automated. Using magnetic-bead technology, DNA can be purified from a variety of clinical samples, while the DNA toxoplasma gondii fragments are analysed with the help of the polymerase chain reaction.
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