IN VITRO, DONE ON STEM CELL CULTURES BIOCOMPATILITY TESTING OF NERVOUS CONDUITS
Abstract:
Extensive nerve injuries, often leading to nerve defects, represent a very challenging
pathology with a complex therapeutic approach: trophic molecules (1 and 2 insulin-like growing factors,
NGF, BDNF, 3, and 4/5neurotrophins), vascularized nerves, metabolic manipulation of pulsating
electric fields. Nowadays, the researches are axed on peripheral nerve tissue engineering, consisting of
viable Schwann or Schwann-like cells implants in nerve conduits manufactured from biocompatible
natural and synthetic polymers. In our study we evaluated polymer biocompatibility, both by qualitative
(Giemsa cell cytochemical staining) and quantitative methods (MTT test). According to the cytotoxicity
scale from the SR EN ISO 10993-5:2003 European standard, it can be concluded that, although the
synthetical polymers are slightly cytotoxic, their mixing with biopolymers led to a significant increase in
viability, thus achieving a good biocompatibility, with the consequent selection of the optimal variants of
polymer shuffles in order to achieve nerve conduits.
full text article in English (.EN) |