IDENTIFICATION OF STAPHYLOCOCCAL ISOLATES FROM BLOOD CULTURES AND INDICATING THE Β-LACTAM RESISTANCE PHENOTYPE USING VITEK® 2 COMPACT AUTOMATED SYSTEM
Abstract:
Blood cultures are one of the most important investigation methods in medical bacteriology. Rapid identification of pathogens and determination of the relevant antibiotic sensitivity profiles are an important step for effective treatment with appropriate antimicrobial agent. Using blood cultures the prognosis of patients can be improved, reducing the acquisition of the antibiotic resistance determinants by bacteria and lowering the total cost of healthcare. Systemic staphylococcal infections caused by community-acquired and the hospital-acquired strains are one of the major causes of mortality worldwide (1). In the last twenty years the increased number of invasive procedures, the widespread use of broad-spectrum antimicrobial agents leads to the emergence of coagulase-negative staphylococci, Staphylococcus epidermidis in particular (2). In our days automated bacteriological systems allows rapid detection of microbial growth in blood cultures. The BACTEC® system (Becton Dickinson Instruments Systems, Sparks, USA) detects directly the CO2 production taking serial samples from the produced gas in the culture bottle (3). The aims of this study are the identification of staphylococcal isolates from blood cultures and the detection of its β-lactam resistance phenotypes with VITEK® 2 COMPACT automated system (bioMerieux, Inc., Hazelwood, USA.) using samples collected during a nearly two-year period in the bacteriology laboratory of the Emergency County Hospital, Sibiu, Romania.
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