ANALYSIS OF DIETARY SUPPLEMENTS AND DRUGS FOR ERECTILE DYSFUNCTION BY HPLC-UV
Abstract:
The popularity of dietary supplements and drugs for erectile dysfunction on the internet,
permissive legal framework in this domain and purchasing these drugs from pharmacy on prescription
only, explain why they are often subject to counterfeit. A HPLC-UV method for the identification and
quantification of sildenafil, tadalafil and vardenafil in dietary supplements and drugs is described.
Separation of these substances was performed on a Phenomenex C8 5μm 150x4.6 mm column, using as
mobile phase a mixture of phase A = 0.1% formic acid in water and phase B = 0.1% formic acid in
acetonitrile, in a concentration gradient, phase A concentration decreasing from 80 to 20% in 15
minutes. The detection was set at 262 nm. The supplements purchased from pharmacies did not contain
phosphodiesterase 5 inhibitors, but in two supplements purchased on the internet, sildenafil was
quantified (13.87 ± 1.79 mg/capsule and 18.34 ± 2.16 mg/capsule). The method was used for the
determination of sildenafil in Viagra® 100 mg tablets purchased online, their content was higher by
35.80% from the declared composition.
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